Conjugation in Chemostat Cultures of Schizosaccharomyces pombe

[Schizosaccharomyces pombe].

Cloning of the Schizosaccharomyces pombe

Diadenosine 5',5"'-P1,P4-tetraphosphate (Ap4A) asymmetric hydrolase (EC 3.6.1.17) is a specific catabolic enzyme of Ap4A found in Schizosaccharomyces pombe. We have previously described the partial purification ofAp4A hydrolase from S. pombe [Robinson, de la Pefia and Barnes (1993) Biochim. Biophys. Acta 1161, 139-148]. We determined the sequence of the N-terminal 20 amino acids of Ap4A hydrola...

Asymmetric inheritance of cytoophidia in Schizosaccharomyces pombe

A general view is that Schizosaccharomyces pombe undergoes symmetric cell division with two daughter cells inheriting equal shares of the content from the mother cell. Here we show that CTP synthase, a metabolic enzyme responsible for the de novo synthesis of the nucleotide CTP, can form filamentous cytoophidia in the cytoplasm and nucleus of S. pombe cells. Surprisingly, we observe that both c...

Dynamics of interphase microtubules in Schizosaccharomyces pombe

BACKGROUND Microtubules in interphase Schizosaccharomyces pombe are essential for maintaining the linear growth habit of these cells. The dynamics of assembly and disassembly of these microtubules are so far uncharacterised. RESULTS Live cell confocal imaging of alpha1 tubulin tagged with enhanced green fluorescent protein revealed longitudinally oriented, dynamically unstable interphase micr...

Modelling of interaction between Schizosaccharomyces pombe and Saccharomyces cerevisiae to predict stable operating conditions in a chemostat

In natural eco systems and industrial operations involving mixed culture systems, interactions among various species greatly influence the performance of the entire bioprocess. The study of the interaction patterns and modelling the process based on it will be of ample importance to obtain optimum operating conditions. Ammensalism is a kind of negative interaction that occurs among diverse micr...